欢迎访问《新能源进展》官方网站!今天是
论文

启动子对木糖异构酶基因在谷氨酸棒杆菌中表达的影响

  • 杨 柳 ,
  • 许敬亮 ,
  • 陈小燕 ,
  • 袁振宏
展开
  • 1. 中国科学院广州能源研究所,广州 510640;
    2. 中国科学院大学,北京 100049
杨 柳(1989-),女,硕士研究生,主要从事生物质能方向研究。

收稿日期: 2014-06-06

  修回日期: 2014-06-23

  网络出版日期: 2014-10-30

基金资助

国家自然科学基金(2117623和21211140237);国家高技术研究发展计划(863计划,2013AA065803);中科院院地合作项目和广州市科技攻关项目(2013J4300026)

Effects of Promoter Modification on Xylose Isomerase Gene xylA Expression in Corynebacterium glutamicum

  • YANG Liu ,
  • XU Jing-liang ,
  • CHEN Xiao-yan ,
  • YUAN Zhen-hong
Expand
  • 1. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China; 
    2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2014-06-06

  Revised date: 2014-06-23

  Online published: 2014-10-30

摘要

以大肠杆菌的强启动子Ptrc和谷氨酸棒杆菌ATCC 13032的内源强启动子Pgro作为大肠杆菌的木糖异构酶基因xylA在谷氨酸棒杆菌中表达的转录起始元件,通过实验表明谷氨酸棒杆菌自身的强启动子Pgro能有效促使外源基因xylA在谷氨酸棒杆菌ATCC 13032中的表达,同时,核糖体结合位点RBS序列的优化对目的基因的表达水平有一定的影响。

本文引用格式

杨 柳 , 许敬亮 , 陈小燕 , 袁振宏 . 启动子对木糖异构酶基因在谷氨酸棒杆菌中表达的影响[J]. 新能源进展, 2014 , 2(5) : 353 -357 . DOI: 10.3969/j.issn.2095-560X.2014.05.005

Abstract

The exogenous gene xylA from E. coli MG1655 was expressed in Corynebacterium glutamicum ATCC 13032 with the strong promoter Ptrc of E. coli and the endogenous promoter Pgro of Corynebacterium glutamicum as transcription start components separately. The experiment result shows that Pgro can be effective in the starting expression xylA in Corynebacterium glutamicum ATCC 13032. In addition, the sequence of RBS also has an impact on the gene expression level.

参考文献

[1] Kawaguchi H. Engineering of a xylose metabolic pathway in Corynebacterium glutamicum[J]. Applied and environmental microbiology, 2006, 72(5): 3418-3428.

[2] 顾方媛. 纤维素酶的研究进展与发展趋势[J]. 微生物学杂志, 2008, 28(1): 83-87.

[3] Jeffries T W. Utilization of xylose by bacteria, yeasts, and fungi[M]. Springer, 1983.

[4] Görke B, and Stülke J. Carbon catabolite repression in bacteria: many ways to make the most out of nutrients[J]. Nature Reviews Microbiology, 2008, 6(8): 613-624.

[5] Yukawa H. Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R[J]. Microbiology, 2007, 153(4): 1042-1058.

[6] Gopinath V, Murali A, Dhar K S, et al. Corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products[J]. Applied Microbiology and Biotechnology, 2011, 93(1): 95-106.

[7] Eggeling L, and Bott M. Handbook of Corynebacterium glutamicum[M]. CRC press, 2010.

[8] Schäfer A, Schwarzer A, Kalinowski J, et al. Cloning and characterization of a DNA region encoding a stress-sensitive restriction system from Corynebacterium glutamicum ATCC 13032 and analysis of its role in intergeneric conjugation with Escherichia coli[J]. Journal of bacteriology, 1994. 176(23): 7309-7319.

[9] Kröger B, Zelder O, Klopprogge C, et al. Pgro expression units[P]. US 2009/0246836 A1, 2009. 1-83.

[10] Pátek M, Nešvera J, Guyonvarch A, et al. Promoters of Corynebacterium glutamicum[J]. Journal of biotechnology, 2003. 104(1): 311-323.

[11] Kawaguchi H, Vertès A A., Okino S, et al. Engineering of a xylose metabolic pathway in Corynebacterium glutamicum[J]. Applied and environmental microbiology, 2006, 72(5): 3418-3428.

[12] 余秉琦, 沈微, 诸葛健, 等. 适用于异源DNA高效整合转化的谷氨酸棒杆菌电转化法[J]. 中国生物工程杂志, 2005, 25(2): 78-81.

[13] Kröger B, Zelder O, Klopprogge C, et al. PEF-TU expression units[P]. US 7785779 B2, 2010, 1-122.

[14] Xu D, Tan Y, Huan X, et al. Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer[J]. Journal of Microbiological Methods, 2010, 80(1): 86-92.

[15] Liu Q, Ouyang S P, Kim J, et al. The impact of PHB accumulation on l-glutamate production by recombinant Corynebacterium glutamicum[J]. Journal of biotechnology, 2007, 132(3): 273-279.

[16] Dische Z. Spectrophotometric method for the determination of free pentose and pentose in nucleotides[J]. Journal of Biological Chemistry, 1949, 181(1): 379-392.

[17] 周世宁, 李甘霖. 嗜热脂肪芽孢杆菌耐热木糖异构酶的特性[J]. 微生物学通报, 1998, 25(5): 271-274.

[18] Primrose S, Twyman R, Old B著. 基因操作原理(第六版)[M]. 瞿礼嘉, 顾红雅译. 北京: 高等教育出版社, 2003.

文章导航

/