启动子对木糖异构酶基因在谷氨酸棒杆菌中表达的影响
收稿日期: 2014-06-06
修回日期: 2014-06-23
网络出版日期: 2014-10-30
基金资助
国家自然科学基金（2117623和21211140237）；国家高技术研究发展计划（863计划，2013AA065803）；中科院院地合作项目和广州市科技攻关项目（2013J4300026）
Effects of Promoter Modification on Xylose Isomerase Gene xylA Expression in Corynebacterium glutamicum
Received date: 2014-06-06
Revised date: 2014-06-23
Online published: 2014-10-30
杨 柳 , 许敬亮 , 陈小燕 , 袁振宏 . 启动子对木糖异构酶基因在谷氨酸棒杆菌中表达的影响[J]. 新能源进展, 2014 , 2(5) : 353 -357 . DOI: 10.3969/j.issn.2095-560X.2014.05.005
The exogenous gene xylA from E. coli MG1655 was expressed in Corynebacterium glutamicum ATCC 13032 with the strong promoter Ptrc of E. coli and the endogenous promoter Pgro of Corynebacterium glutamicum as transcription start components separately. The experiment result shows that Pgro can be effective in the starting expression xylA in Corynebacterium glutamicum ATCC 13032. In addition, the sequence of RBS also has an impact on the gene expression level.
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