四种能源草中黄酮类物质的定性研究
收稿日期: 2016-04-26
修回日期: 2016-06-12
网络出版日期: 2016-06-27
基金资助
国家科技支撑计划（2013BAD22B03）；
广州市科技计划项目（201508020098）
Determination of Flavonoids from Four Energy Grasses
Received date: 2016-04-26
Revised date: 2016-06-12
Online published: 2016-06-27
本文对杂交狼尾草、柳枝稷、芦竹及象草这4种能源草的整株、茎和叶中的黄酮类物质总量进行了定量分析，并利用高效液相色谱质谱联用技术对黄酮物质种类进行定性分析。对整株分析的结果表明，柳枝稷的总黄酮含量最高，达到6.84 mg当量槲皮素/g干重，比芦竹、杂交狼尾草和象草分别高出59.8%、20.1%和11.2%。能源草的不同部位黄酮分布存在差异，叶中黄酮含量是茎的2.3 ~ 3.0倍。杂交狼尾草叶中含量最高，为10.55 mg当量槲皮素/g干重。定性分析发现，4种能源草的提取物中共含有14种黄酮苷和2种黄酮醇，包括槲皮苷、异鼠李素和槲皮素。该研究结果可为能源草作为天然产物提取原料提供理论支持。
杨立贵 , 孙永明 , 孔晓英 , 李连华 , 刘姝娜 , 董鹏宇 , 袁振宏 . 四种能源草中黄酮类物质的定性研究[J]. 新能源进展, 2016 , 4(3) : 225 -231 . DOI: 10.3969/j.issn.2095-560X.2016.03.010
The total flavonoid contents in four common energy grasses (hybrid Pennisetum, switchgrass, giant reed, elephant grass) including whole strain, stems and leaves were quantified. The flavonoids were detected by LC-MS. For the whole strains, switchgrass has a flavonoids content of 6.84 mg quercetin equivalents/g dry weight, which is 59.8%, 20.1% and 11.2% higher than giant reed, hybrid Pennisetum and elephant grass. The content in leaves is 2.3 ~ 3.0 times to stems, the contents in hybrid Pennisetum leaves is 10.55 mg quercetin equivalents/g dry weight, which is the highest among all these samples. 14 flavonoid glycosides and 2 flavonols were identified in the extracts of four grasses. Quercetin, isorhamnetin and quercitrin are identified positively with standards. This research may provide a theoretical support for the value added phytochemicals extract from energy grass.
Key words： energy grass; LC-MS; flavonoid glycosides; flavonol; total flavonoid content
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