一株脱硫菌株的分离鉴定及其对硫化物的去除效果验证
收稿日期: 2016-09-14
修回日期: 2016-10-21
网络出版日期: 2016-12-28
基金资助
国家科技支撑计划项目（2015BAD21B01）
Isolation and Identification of a Desulfurization Strain and Its Sulfide Removal Performance
Received date: 2016-09-14
Revised date: 2016-10-21
Online published: 2016-12-28
为获得适合生物脱硫工程应用的硫氧化细菌，取污水处理厂曝气池活性污泥，采用人工模拟硫化物废水作为硫源富集培养并经分离纯化，得到一株能够高效快速去除废水中硫化物的硫氧化细菌菌株DS-7。该菌为短杆状、长0.8 ~ 2 μm、宽0.6 ~ 0.8 μm、革兰氏阴性，最适生长温度为30℃，最适生长pH值为7.0。通过对该菌的16SrDNA序列测定，并结合其形态和生理特性鉴定，该菌株属于鞘氨醇杆菌属（Sphingobacterium）。Sphingobacterium sp. DS-7去除硫化物性能研究结果表明：当硫化物浓度为650 mg/L时，该菌的4 h硫化物去除率达到95%以上，相应的硫转化率最高达到45.08%，生成的单质硫颗粒形状为不规则八面体。
赵 鹏 , 李 东 , 周一民 , 刘晓风 , 廖银章 . 一株脱硫菌株的分离鉴定及其对硫化物的去除效果验证[J]. 新能源进展, 2016 , 4(6) : 425 -430 . DOI: 10.3969/j.issn.2095-560X.2016.06.001
In order to obtain sulfur-oxidizing bacteria for engineering application of biodesulfurization, the enrichment and acclimation of aerobic activated sludge was carried out by using artificial sulfide wastewater as a sulfur source. A sulfur-oxidizing bacteria strain DS-7 with strong ability of sulfide removal was isolated from the enrichment culture. The strain DS-7 was Gram-negative and short rod shaped with length of 0.8 ~ 2 μm and width of 0.6 ~ 0.8 μm. The optimal growth temperature and pH was 30oC and 7.0, respectively. The identified strain DS-7 belongs to the genus of Sphingobacterium according to the 16SrDNA bacterial sequences and the morphological and physiological characterization. The sulfide removal performance tests of Sphingobacterium sp. DS-7 showed that the sulfide removal rate was more than 95% and the sulfur conversion rate reached 45.08% after culturing for 4 h at the initial sulfide concentration of 650 mg/L. The sulfur formed by Sphingobacterium sp. DS-7 was in the shape of irregular octahedron.
Key words： sulfur-oxidizing bacteria; biodesulfurization; sulfide; 16SrDNA; identification
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