脂溶性荧光染料测定微生物油脂的研究进展

赵  成; 陈雪芳; 熊  莲; 郭海军; 黄前霖; 黄  超; 陈新德

doi:10.3969/j.issn.2095-560X.2019.01.009

新能源进展 >

2019 , Vol. 7 >Issue 1: 85 - 92

DOI: https://doi.org/10.3969/j.issn.2095-560X.2019.01.009

论文

脂溶性荧光染料测定微生物油脂的研究进展

赵成 ,
陈雪芳 ,
熊莲 ,
郭海军 ,
黄前霖 ,
黄超 ,
陈新德

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1. 中国科学院广州能源研究所，广州 510640；
2. 中国科学院可再生能源重点实验室，广州 510640；
3. 广东省新能源和可再生能源研究开发与应用重点实验室，广州 510640；
4. 中国科学院大学，北京 100049

赵成（1992-），男，硕士研究生，主要从事生物质能研究。陈新德（1967-），男，教授级高级工程师，博士生导师，主要从事生物质能研究。E-mail：chenxd@ms.giec.ac.cn 黄超（1983-），男，博士，副研究员，硕士生导师，主要从事生物质能研究。E-mail：huangchao@ms.giec.ac.cn

收稿日期: 2018-07-17

修回日期: 2018-08-05

网络出版日期: 2019-02-27

基金资助

国家自然科学基金项目（21606229，51378486）；广州市珠江科技新星专项项目（201610010014，201806010107）；中科院可再生能源重点实验室基金项目（Y707j41001）

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Research Progress of Determining Microbial Lipid by Liposoluble Fluorescent Probe

ZHAO Cheng ,
CHEN Xue-fang ,
XIONG Lian ,
GUO Hai-jun ,
HUANG Qian-Lin ,
HUANG Chao ,
CHEN Xin-de

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1. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China;
2. CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China;
3. Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China;
4. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2018-07-17

Revised date: 2018-08-05

Online published: 2019-02-27

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摘要

微生物油脂因生产不受气候、季节影响，占地少，所需人力较少等优点，被认为是生物柴油的理想原料。不同种类的油脂微生物的脂质含量差别较大，因此，高效筛选油脂含量高的微生物菌株是开发微生物油脂资源的关键问题，而如何分析、测定微生物的油脂含量是筛选高油脂含量菌株不可缺少的步骤。荧光分析法具有速度快、污染少、可原位测定等优势正逐渐取代传统氯仿/甲醇重量分析方法，被广泛应用于定性定量分析微生物油脂。本文综述了脂溶性荧光染料（以尼罗红与BODIPY 505/515为主）测定微生物油脂的研究进展，以及其应用中存在的问题，并提出了未来改进该方法的研究思路。

关键词： 荧光探针; 尼罗红; BODIPY 505/515; 微生物油脂

本文引用格式

赵成 , 陈雪芳 , 熊莲 , 郭海军 , 黄前霖 , 黄超 , 陈新德 . 脂溶性荧光染料测定微生物油脂的研究进展[J]. 新能源进展, 2019 , 7(1) : 85 -92 . DOI: 10.3969/j.issn.2095-560X.2019.01.009

Abstract

Microbial lipid was deemed as an ideal raw material to produce biodiesel, possessing characteristics of no limit of season and climate, occupation less land space and manpower, etc. The lipid contents vary with different strains, so the key factor for developing the source of microbial lipid is to screen the microbial species with high lipid content. The essential step for screening the strains with high lipid content is to analyze and measure the lipid content of microorganism. Fluorescent method, which is quick, less pollution, measuring in stiu, have been applied to analyze microbial lipid qualitatively and quantitatively, is now gradually replacing the traditional gravimetric method by chloroform/methyl. This review narrated the development and some problems of microbial lipid analyzed by liposoluble fluorescent probe (mianly Nile red and BODIPY 505/515). Also, some research means for developing this method were put forward.

Key words： fluorescent probe; Nile red; BODIPY 505/515; microbial lipid

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