甘蔗渣的不同预处理方法比较及其酶水解的类分形动力学

张宇; 许敬亮; 袁振宏; 刘云云; 何敏超

doi:10.3969/j.issn.2095-560X.2013.02.007

新能源进展 >

2013 , Vol. 1 >Issue 2: 166 - 169

DOI: https://doi.org/10.3969/j.issn.2095-560X.2013.02.007

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甘蔗渣的不同预处理方法比较及其酶水解的类分形动力学

张宇 ,
许敬亮 ,
袁振宏 ,
刘云云 ,
何敏超

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中国科学院广州能源研究所，中国科学院可再生能源重点实验室，广州 510640

张宇（1982-），男，博士，副研究员，中国科学院广州能源研究所研究助理，主要从事纤维素乙醇研究。

收稿日期: 2013-08-08

修回日期: 2013-08-21

网络出版日期: 2013-10-31

基金资助

国家高新技术发展计划（2013AA065803）；中国科学院广州能源研究所所长创新基金（y007rd1001）

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Comparison of Different Pretreatment Methods on Sugarcane Bagasse and Fractal-like Kinetics of Enzymatic Hydrolysis

ZHANG Yu ,
XU Jing-liang ,
YUAN Zhen-hong ,
LIU Yun-yun ,
HE Min-chao

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CAS Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China

Received date: 2013-08-08

Revised date: 2013-08-21

Online published: 2013-10-31

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摘要

分别采用NaOH（0.45 mol/L aq.）、HCl（0.034 mol/L aq.）和高温液态水（LHW）三种方法对甘蔗渣进行预处理，并对其组分变化和酶解效果进行了比较。NaOH预处理方法获得最高的木质素去除率，达91.1%，糖损失率达23.5%；HCl和LHW预处理结果类似，木聚糖溶解率分别为85.2%和79.7%，糖损失率均约为15%，木质素去除率均小于16%。三种方法处理的甘蔗渣经纤维素酶水解后得到总单糖（葡萄糖 + 木糖）浓度分别为38.7 g/L（NaOH）、16.1 g/L（HCl）和15.6 g/L（LHW）。综合比较预处理和酶水解工艺，NaOH水溶液预处理法的糖回收率最高，其次为HCl水溶液预处理法，LHW预处理法的糖回收率最低。作为描述纤维素酶反应动力学的有力工具，类分形理论的研究表明，各种预处理后底物的不规则性依次为：HCl＞LHW＞NaOH，其与酶的有效吸附大小依次为：NaOH＞HCl＞LHW。

关键词： 甘蔗渣; 预处理; 纤维素酶; 类分形动力学; 生物质能

本文引用格式

张宇 , 许敬亮 , 袁振宏 , 刘云云 , 何敏超 . 甘蔗渣的不同预处理方法比较及其酶水解的类分形动力学[J]. 新能源进展, 2013 , 1(2) : 166 -169 . DOI: 10.3969/j.issn.2095-560X.2013.02.007

Abstract

NaOH (0.45 mol/L aq.), HCl (0.034 mol/L aq.) and LHW (liquid hot water) were used to pretreat bagasse, and the composition change and enzymatic saccharification were compared. NaOH pretreatment brought the highest lignin removal (91.1%) and 23.5% sugar loss; the similar composition variation occurred for HCl and LHW pretreatment, where the xylan dissolution were 85.2% and 79.7%, respectively. The sugar loss and lignin removal was both about 15% and less than 16%, respectively. After enzymatic saccharification, the total obtained sugar (glucose + xylose) concentration were 38.7, 16.1 and 15.6 g/L from NaOH, HCl and LHW pretreated sugarcane bagasse, respectively. The total sugar yield including the whole pretreatment and saccharification was: NaOH > HCl > LHW. As a persuasive tool, the fractal-like theory was used to study cellulase kinetics. Results showed that the irregularity of pretreated bagasse was: HCl > LHW > NaOH, and the absorbability was: NaOH > HCl > LHW.

Key words： sugarcane bagasse; pretreatment; cellulase; fractal-like kinetics; bioenergy

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