富含碳水化合物微藻的筛选、鉴定及其在不同氮浓度条件下的产糖分析
收稿日期: 2015-07-13
修回日期: 2015-08-27
网络出版日期: 2015-10-30
基金资助
国家高技术研究发展计划（863计划， 2013AA065803）；
国家自然科学基金（2117623，21211140237）；
广东省科技攻关项目（2013B010403021）；
广州市科技攻关项目（2013J4300026
Screening and Identification of Carbohydrate-rich Microalgae and Carbohydrate Production under Different Concentrations of Nitrogen
Received date: 2015-07-13
Revised date: 2015-08-27
Online published: 2015-10-30
肖仕圆，许敬亮，袁 涛，赵 月，吴 浩，李谢昆，袁振宏 . 富含碳水化合物微藻的筛选、鉴定及其在不同氮浓度条件下的产糖分析[J]. 新能源进展, 2015 , 3(5) : 340 -345 . DOI: 10.3969/j.issn.2095-560X.2015.05.004
The growth rate and starch content of four strains of microalgae preserved in our laboratory were analyzed. A carbohydrate-rich microalgae #3, as one of the four, was screened. Its growth rate and starch content could attain to 0.086 g/(L•d) and 225.2 mg/L, respectively. Further, microalgae #3 was identified as Desmodesmus sp. based on 18s rDNA cluster analysis. The growth rate of Desmodesmus sp. #3 was low and the total sugar accumulation was fast when cultured without adding nitrogen. The growth rate change at different nitrogen concentration was almost similar, but total sugar accumulation speed presented different change tendency.
Key words： microalgae; 18S rDNA; total sugar
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