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Screening and Identification of Carbohydrate-rich Microalgae and Carbohydrate Production under Different Concentrations of Nitrogen
Received date: 2015-07-13
Revised date: 2015-08-27
Online published: 2015-10-30
The growth rate and starch content of four strains of microalgae preserved in our laboratory were analyzed. A carbohydrate-rich microalgae #3, as one of the four, was screened. Its growth rate and starch content could attain to 0.086 g/(L•d) and 225.2 mg/L, respectively. Further, microalgae #3 was identified as Desmodesmus sp. based on 18s rDNA cluster analysis. The growth rate of Desmodesmus sp. #3 was low and the total sugar accumulation was fast when cultured without adding nitrogen. The growth rate change at different nitrogen concentration was almost similar, but total sugar accumulation speed presented different change tendency.
Key words： microalgae; 18S rDNA; total sugar
XIAO Shi-yuan1,2, XU Jing-liang1, YUAN Tao1,2, ZHAO Yue1,2, WU Hao1, LI Xie-kun1,2, YUAN Zhen-hong1 . Screening and Identification of Carbohydrate-rich Microalgae and Carbohydrate Production under Different Concentrations of Nitrogen[J]. Advances in New and Renewable Energy, 2015 , 3(5) : 340 -345 . DOI: 10.3969/j.issn.2095-560X.2015.05.004
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