Advances in New and Renewable Energy >
Isolation and Identification of a Desulfurization Strain and Its Sulfide Removal Performance
Received date: 2016-09-14
Revised date: 2016-10-21
Online published: 2016-12-28
In order to obtain sulfur-oxidizing bacteria for engineering application of biodesulfurization, the enrichment and acclimation of aerobic activated sludge was carried out by using artificial sulfide wastewater as a sulfur source. A sulfur-oxidizing bacteria strain DS-7 with strong ability of sulfide removal was isolated from the enrichment culture. The strain DS-7 was Gram-negative and short rod shaped with length of 0.8 ~ 2 μm and width of 0.6 ~ 0.8 μm. The optimal growth temperature and pH was 30oC and 7.0, respectively. The identified strain DS-7 belongs to the genus of Sphingobacterium according to the 16SrDNA bacterial sequences and the morphological and physiological characterization. The sulfide removal performance tests of Sphingobacterium sp. DS-7 showed that the sulfide removal rate was more than 95% and the sulfur conversion rate reached 45.08% after culturing for 4 h at the initial sulfide concentration of 650 mg/L. The sulfur formed by Sphingobacterium sp. DS-7 was in the shape of irregular octahedron.
Key words： sulfur-oxidizing bacteria; biodesulfurization; sulfide; 16SrDNA; identification
ZHAO Peng , LI Dong , ZHOU Yi-min , LIU Xiao-feng , LIAO Yin-zhang . Isolation and Identification of a Desulfurization Strain and Its Sulfide Removal Performance[J]. Advances in New and Renewable Energy, 2016 , 4(6) : 425 -430 . DOI: 10.3969/j.issn.2095-560X.2016.06.001
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