Xylanase producing fungi was isolated and identified, and its optimum condition for solid state fermentation and enzymatic properties were studied. The genealogical classification was analyzed by observation of colony morphology and ITS gene sequence. The optimal xylanase-producing conditions were assayed by single factor experiments. The results indicated that the isolated strain with high yielding xylanase was identified as Aspergillus niger. Its optimum xylanase activity was 10 446.92 IU/g under conditions as follows: 1:3 as the ratio between corncob and wheat bran, urea as nitrogen source, 5.0 as initial pH, 1:3.5 as ratio between solid and liquid, 10.0% as inoculum size. Study of xylanase charateristics showed that enzymatic activity were the optimum at pH of 5.0 and temperature of 45oC. Addition of furfural (23.0 g/L) or hydroxymethylfurfural (25.7 g/L) plays an activating effect with an increase of 15.9% and 18.4%, respectively. Therefore, Aspergillus niger SM751 could to be a promising microorganism for the production of xylanase, which could be used in the field of enzymatic hydrolysis of lignocellulose.
HE Min-chao
,
YAN Zhi-ying
,
ZHANG Jin-feng
,
LIU Yun-yun
,
CHEN Xiao-yan
,
XU Jing-liang
. Screening of Strain with High-Level of Xylanase, Enzymatic Characters and Hydrolysis Application[J]. Advances in New and Renewable Energy, 2019
, 7(4)
: 318
-324
.
DOI: 10.3969/j.issn.2095-560X.2019.04.004
[1] 温博婷, 孙丽超, 王凤忠, 等. 微生物木聚糖酶的研究进展及其在食品领域的应用[J]. 生物产业技术, 2017(5): 81-86. DOI:10.3969/j.issn.1674-0319.2017.05.014.
[2] 陈阳雷, 吉兴香, 许凤. 纸浆木聚糖酶生物助漂技术进展[J]. 中国造纸, 2017, 36(12): 74-78. DOI: 10.11980/j.issn.0254-508X.2017.12.013.
[3] KUMAR V, CHHABRA D, SHUKLA P.Xylanase production from Thermomyces lanuginosus VAPS-24 using low cost agro-industrial residues via hybrid optimization tools and its potential use for saccharification[J]. Bioresource technology, 2017, 243: 1009-1019. DOI: 10.1016/j.biortech.2017.07.094.
[4] HU J G, ARANTES V, SADDLER J N.The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect?[J]. Biotechnology for biofuels, 2011, 4: 36. DOI: 10.1186/1754-6834-4-36.
[5] QING Q, YANG B, WYMAN C E.Xylooligomers are strong inhibitors of cellulose hydrolysis by enzymes[J]. Bioresource technology, 2010, 101(24): 9624-9630. DOI: 10.1016/j.biortech.2010.06.137.
[6] ZHANG J H, TANG M, VIIKARI L.Xylans inhibit enzymatic hydrolysis of lignocellulosic materials by cellulases[J]. Bioresource technology, 2012, 121: 8-12. DOI: 10.1016/j.biortech.2012.07.010.
[7] 何敏超, 张宇, 许敬亮, 等. 木聚糖酶高产菌筛选研究进展[J]. 化工进展, 2013, 32(1): 145-150. DOI:10.3969/j.issn.1000-6613.2013.01.024.
[8] 王停停, 郑宏臣, 徐健勇, 等. 木聚糖酶XynG1-1在毕赤酵母中的表达及发酵条件优化[J]. 食品与发酵工业, 2017, 43(1): 37-43. DOI: 10.13995/j.cnki.11-1802/ts.201701007.
[9] 侯洁, 李琴, 李秀婷, 等. 产木聚糖酶霉菌发酵条件优化及酶学性质研究[J]. 食品工业, 2016, 37(10): 130-134.
[10] KIM Y, XIMENES E, MOSIER N S, et al.Soluble inhibitors/deactivators of cellulase enzymes from lingo- cellulosic biomass[J]. Enzyme and microbial technology, 2011, 48(4/5): 408-415. DOI: 10.1016/j.enzmictec.2011.01.007.
[11] BOUKARI I, O’DONOHUE M, RÉMOND C, et al. Probing a family GH11 endo-β-1, 4-xylanase inhibition mechanism by phenolic compounds: Role of functional phenolic groups[J]. Journal of molecular catalysis B: enzymatic, 2011, 72(3/4): 130-138. DOI: 10.1016/j. molcatb.2011.05.010.
[12] MANDELS M, WEBER J, PARIZEK R.Enhanced cellulase production by a mutant of Trichoderma viride[J]. Applied microbiology, 1971, 21(1): 152-154. DOI: org/10.1016/0141-0229(94)00131.
[13] BAILEY M J, BIELY P, POUTANEN K.Interlaboratory testing of methods for assay of xylanase activity[J]. Journal of biotechnology, 1992, 23(3): 257-270. DOI: 10.1016/0168-1656(92)90074-J.
[14] BETINI J H A, MICHELIN M, PEIXOTO-NOGUEIRA S C, et al. Xylanases from Aspergillus niger, Aspergillus niveus and Aspergillus ochraceus produced under solid- state fermentation and their application in cellulose pulp bleaching[J]. Bioprocess and biosystems engineering, 2009, 32(6): 819-824. DOI: 10.1007/s00449-009-0308-y.
[15] MCINTOSH S, VANCOV T.Optimisation of dilute alkaline pretreatment for enzymatic saccharification of wheat straw[J]. Biomass and bioenergy, 2011, 35(7): 3094-3103. DOI: 10.1016/j.biombioe.2011.04.018.
[16] YU Q, ZHUANG X S, LV S L, et al.Liquid hot water pretreatment of sugarcane bagasse and its comparison with chemical pretreatment methods for the sugar recovery and structural changes[J]. Bioresource technology, 2013, 129: 592-608. DOI: 10.1016/j.biortech.2012.11.099.
[17] DE SOUZA MOREIRA L R, DE CARVALHO CAMPOS M, DE SIQUEIRA P H V M, et al. Two β-xylanases from Aspergillus terreus: Characterization and influence of phenolic compounds on xylanase activity[J]. Fungal genetics and biology, 2013, 60: 46-52. DOI: 10.1016/j.fgb.2013.07.006.
[18] BERLIN A, BALAKSHIN M, GILKES N, et al.Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations[J]. Journal of biotechnology, 2006, 125(2): 198-209. DOI: 10.1016/j.jbiotec.2006.02.021.
[19] TIAN Y, JIANG Y, OU S Y.Interaction of cellulase with three phenolic acids[J]. Food chemistry, 2013, 138(2/3): 1022-1027. DOI: 10.1016/j.foodchem.2012.10.129.
[20] LI J B, ZHOU P F, LIU H M, et al.Synergism of cellulase, xylanase, and pectinase on hydrolyzing sugarcane bagasse resulting from different pretreatment technologies[J]. Bioresource technology, 2014, 155: 258-265. DOI: 10.1016/j.biortech.2013.12.113.
[21] KAYA F, HEITMANN J A, JOYCE T W.In?uence of lignin and its degradation products on enzymatic hydrolysis of xylan[J]. Journal of biotechnology, 2000, 80(3): 241-247. DOI: 10.1016/S0168-1656(00)00265-0.
[22] VAN DYK J S, SAKKA M, SAKKA K, et al. Characterisation of the multi-enzyme complex xylanase activity from Bacillus licheniformis SVD1[J]. Enzyme and microbial technology, 2010, 47(4): 174-177. DOI: 10.1016/j.enzmictec.2010.06.004.
